Effects of ethanol on CYP2E1 levels and related oxidative stress using a standard balanced diet

Expression of cytochrome P4502E1 (CYP2E1) is very much influenced by nutritional factors, especially carbohydrate consumption, and various results concerning the expression of CYP2E1 were obtained with a low-carbohydrate diet. This study describes the effects of ethanol treatment on CYP2E1 levels and its relationship with oxidative stress using a balanced standard diet to avoid low or high carbohydrate consumption. Rats were fed for 1, 2, 3, or 4 weeks a commercial diet plus an ethanol-sucrose solution. the results have shown that ethanol administration was associated with CYP2E1 induction and stabilization without related oxidative stress. Our findings suggest that experimental models with a low-carbohydrate/high-fat diet produce some undesirable CYP2E1 changes that are not present when a balanced standard diet is given.Universidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilFac Med ABC, Dept Hematol & Oncol, São Paulo, BrazilUniv Anhembi Morumbi, São Paulo, BrazilUniv Chile, Fac Med, Programa Farmacol Mol & Clin, Inst Ciencias Biomed, Santiago 7, ChileUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilWeb of Scienc