Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues
- Soares, Rodrigo Pedro
- Torrecilhas, Ana Claudia Trocoli
- Assis, Rafael Ramiro de
- Rocha, Marcele Neves
- Castro, Felipe Augusto Moura e
- Freitas, Gustavo Ferreira de
- Murta, Silvane Maria
- Santos, Sara Lopes dos
- Marques, Alexandre Ferreira
- Almeida, Igor Correia de
- Romanha, Alvaro Jose
DOIAbstract
The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. the significance of these variations was assessed after analysis of the terminal alpha-galactosyl residues. Enzymatic treatment with alpha-galactosidase indicated a differential expression of O-linked alpha-galactosyl residues among the s...
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