Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide

P>AimsTo evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo.MethodologyAliquots of 1 x 104 murine fibroblasts were incubated at 37 degrees C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 mu g mL-1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one-way anova followed by Tukey's test.ResultsMineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single-cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement.ConclusionWhite MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Leopoldo Mand Univ, Dent Res Ctr, Endodont Area, Campinas, SP, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilFAPESP: 07/01228-4CNPq: 561357/2008-0Web of Scienc