Proinflammatory and Anti-inflammatory Cytokines Present in the Acute Phase of Experimental Colitis Treated with Saccharomyces boulardii

To study the proinflammatory and anti-inflammatory cytokines present in the acute phase of trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis treated with Saccharomyces boulardii.Thirty male Wistar rats were divided into three groups: (1) treated group-received Saccharomyces boulardii for 14 days; (2) non-treated group-received sodium chloride solution for 14 days; (3) control group. Colitis was induced on the seventh day of the study in the treated and the non-treated groups using TNBS (10 mg) dissolved in 50% ethanol. Quantification of cytokines, including interleukin (IL)-1 beta (IL-1 beta), IL-6, transforming growth factor-beta (TGF-beta), IL-10 and tumor necrosis factor-alpha (TNF-alpha), in the serum and colonic tissue collected on day 14 were carried out using an enzyme-linked immunosorbent assay (ELISA).The mean concentrations of TGF-beta in both the serum and the colonic tissue of the treated group were statistically higher than that of the control group. the mean concentration of TGF-beta in the colonic tissue of the non-treated group was also statistically higher than the control group.The group treated with Saccharomyces boulardii showed increased amounts of TGF-beta, an anti-inflammatory cytokine, during the acute phase of colitis. There were no differences in the amount of TNF-alpha, IL-1 beta, IL-6, and IL-10 between the treated and the non-treated or the control groups during the acute phase of experimental colitis induced by TNBS.Sao Leopoldo Mand Dent Res Ctr, Campinas, SP, BrazilUniversidade Federal de São Paulo, Div Pediat Gastroenterol, São Paulo, BrazilUniversidade Federal de São Paulo, Div Pediat Gastroenterol, São Paulo, BrazilWeb of Scienc