Single embryo and oocyte lipid fingerprinting by mass spectrometry
- Ferreira, Christina R.
- Saraiva, Sergio A.
- Catharino, Rodrigo R.
- Garcia, Jerusa S.
- Gozzo, Fabio C.
- Sanvido, Gustavo B.
- Santos, Luiz Fernando A.
- Lo Turco, Edson G.
- Pontes, Jose Henrique F.
- Basso, Andrea C.
- Bertolla, Ricardo P.
- Sartori, Roberto
- Guardieiro, Monique M.
- Perecin, Felipe
- Meirelles, Flvio V.
- Sangalli, Juliano R.
- Eberlin, Marcos N.
DOIAbstract
Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI...
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