Is the geographical origin of a Paracoccidioides brasiliensis isolate important for antigen production for regional diagnosis of paracoccidioidomycosis?

P>In this study, exoantigens produced from two Paracoccidioides brasiliensis strains isolated in two different geographical areas were compared in terms of sensitivity and specificity in relation to paracoccidioidomycosis (PCM) diagnosis. Exoantigens from P. brasiliensis 550B (Ag 550B) isolated in the central-west region of Brazil (Mato Grosso State) and exoantigen produced from P. brasiliensis B-339 (Ag B-339) used in reference laboratories were compared by immunodiffusion (ID) tests. When Ag 550B was used in ID test against sera of patients from Mato Grosso and São Paulo, positivity was 92.3% and 41.3%, respectively. On the other hand, when Ag B-339 was tested with the same sera, positivity was 26.2% and 100%, respectively. These results suggest that differences in the antigenic composition probably related to phylogenetic peculiarities in P. brasiliensis isolates from the central-western region of Brazil should be considered in the diagnosis of PCM.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Mato Grosso, Fac Ciencias Med, Sch Med Sci, Mycol Lab, BR-78060900 Cuiaba, MG, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilAdolfo Lutz Inst, Immunol Sect, Mycoses Immunodiagnost Lab, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of Scienc