Association of PPAR alpha gene polymorphisms and lipid serum levels in a Brazilian elderly population

Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a nuclear transcription factor strictly involved in lipid and lipoprotein metabolisms. Thus, PPAR alpha gene polymorphisms have been investigated as cardiovascular risk factors. We aimed to investigate associations of L162V and intron 7G>C polymorphisms with common morbidities affecting a Brazilian elderly cohort as well as with lipid and protein serum levels. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP), and allele frequencies were determined. in addition, we performed the linkage disequilibrium, analysis. Descriptive statistics, logistic regression analysis, and Student's t-test were used. Rare alleles for L162V and intron 7 G>C polymorphisms showed frequencies of 0.047 and 0.199, respectively. Our data showed that these polymorphisms were in linkage disequilibrium (p=0.0002). Intron 7 G>C polymorphism presented a tendency of association with neoplasia (p=0.053), and C allele was associated with higher HDL (p=0.010), lower triglycerides (p=0.001), and VLDL levels (p=0.003) compared to G allele. These data might suggest a protective role of intron 7 G>C polymorphism, in the development of cardiovascular diseases and will help to clarify the importance of PPARa polymorphisms as key modulators of lipid metabolism in Brazilian population. (C) 2009 Elsevier Inc. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, Disciplina Genet,Genet Div,UNIFESP, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Geriatr, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, Disciplina Genet,Genet Div,UNIFESP, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Geriatr, São Paulo, BrazilWeb of Scienc