Chemical Composition and Antifungal Activity of Essential Oil from Brazilian Propolis

The essential oil from Brazilian propolis was isolated by hydrodistillation in a yield of 0.04 %. Twenty-five compounds were identified by GC-MS and calculation of their Kovats indexes. the most abundant essential oil components were alpha-pinene (18.3 %), beta-pinene (6.5 %), germacrene D (6.4 %) and delta-cadinene (7.0 %). Additionally, the antifungal potential of crude oil was evaluated using a bioautographic assay. the minimal amount required to inhibit the growth of fungi Cladosporium cladosporioides and C. sphaerospermum assay was determined as 5.0 mu g, in comparison to positive control nystatin (1.0 mu g) and miconazole (1.0 mu g).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Presbiteriana Mackenzie, Ctr Ciencias & Humanidades, BR-01302907 São Paulo, BrazilInst Bot, Secao Fisiol & Bioquim Plantas, BR-10051 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilWeb of Scienc