Lycopene activity against chemically induced DNA damage in Chinese hamster ovary cells

Lycopene is a natural pigment synthesized by plants and microorganisms, and it is mainly found in tomatoes. It is an acyclic isomer of P-carotene and one of the most potent antioxidants. Several studies have demonstrated the ability of lycopene to prevent chemically induced DNA damage; however, the mechanisms involved are still not clear. in the present study, we investigated the antigenotoxic/antimutagenic effects of lycopene in Chinese Hamster Ovary Cells (CHO) treated with hydrogen peroxide, methylmethanesulphonate (MMS), or 4-nitroquinoline-1-oxide (4-NQO). Lycopene (97%), at final concentrations of 10, 25, and 50 M, was tested under three different protocols: before, simultaneously, and after the treatment with the mutagens. Comet and cytokinesis-block micronucleus assays were used to evaluate the level of DNA damage. Data showed that lycopene reduced the frequency of micronucleated cells induced by the three mutagens. However, this chemopreventive activity was dependent on the concentrations and treatment schedules used. Similar results were observed in the comet assay, although some enhancements of primary DNA damage were detected when the carotenoid was administered after the mutagens. in conclusion, our findings confirmed the chemopreventive activity of lycopene, and showed that this effect occurs under different mechanisms. (c) 2007 Elsevier B.V. All rights reserved.São Paulo State Univ, Botucatu Med Sch, Dept Pathol, BR-18518000 São Paulo, BrazilSão Paulo State Univ, Inst Biosci, Botucatu, SP, BrazilSão Paulo State Univ, Botucatu Med Sch, Dept Clin Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Hlth Sci, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Hlth Sci, São Paulo, BrazilWeb of Scienc