Annexin 1: differential expression in tumor and mast cells in human larynx cancer

Annexin 1 protein (ANXA1) expression was evaluated in tumor and mast cells in human larynx cancer and control epithelium. the effect of the exogenous ANXA1 (peptide Ac 2-26) was also examined during the cellular growth of the Hep-2 human larynx epidermoid carcinoma cell line. This peptide inhibited the proliferation of the Hep-2 cells within 144 hr. in surgical tissue specimens from 20 patients with larynx cancer, ultrastructural immunocytochemistry analysis showed in vivo down-regulation of ANXA1 expression in the tumor and increased in mast cells and Hep-2 cells treated with peptide Ac2-26. Combined in vivo and in vitro analysis demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth. We believe that a better understanding of the regulatory mechanisms of ANXA1 in tumor and mast cells may lead to future biological targets for the therapeutic intervention of human larynx cancer. (c) 2007 Wiley-Liss, Inc.Univ Estadual Paulista, IBILCE, Dept Biol, BR-15054000 São Paulo, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilFac Med, Dept Mol Biol, São Paulo, BrazilFac Med, Dept Pathol, São Paulo, BrazilFac Med, Dept Otorhinolaryngol, São Paulo, BrazilUniv Oxford, Dept Anat & Genet, Oxford, EnglandUniversidade Federal de São Paulo, São Paulo, BrazilWeb of Scienc