Antidepressant-like effect of Cecropia glazioui Sneth and its constituents - in vivo and in vitro characterization of the underlying mechanism

The present study aimed to characterize the antidepressant-like effect of a standardized aqueous extract (AE) of Cecropia glazioui Sneth and its purified fractions on in vivo (forced swimming test), ex vivo (hippocampal monoamines levels) and in vitro (serotonin, noradrenaline and dopamine uptake) tests, searching for the active principles and the underlying mechanisms of action. Treatment with AE, or with its butanolic fraction (BuF), the latter rich in catechins, procyanidins and flavonoids, reduced the immobility of rats in the forced swimming test indicating an antidepressant-like effect. Biochemical analysis of the hippocampal neurotransmitters in BuF-treated rats showed significant increase in monoamines levels. BuF and six of its purified constituents inhibited the uptake of [H-3]-serotonin, [H-3]-dopamine and [H-3]-noradrenaline by synaptosomes of different brain regions. Catechin, catechin (4 alpha -> 8) ent-catechin (Procyanidin B3 isomer) and epicatechin (4 beta -> 8) epicatechin (Procyanidin B2) were the most active compounds. Comparatively, the uptake of [H-3]-noradrenaline was the most affected. These results show that the antidepressant-like effect promoted by C. glazioui extract is most likely due to the blockade of the monoamines uptake in the CNS. (C) 2007 Elsevier GmbH. All rights reserved.Universidade Federal de São Paulo, Dept Pharmacol, Nat Prod Sect, Escola Paulista Med, BR-04044020 São Paulo, BrazilUniv Fed Santa Catarina, CCB, Dept Pharmacol, Neuropharmacol Lab, BR-88015420 Florianopolis, SC, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, Nat Prod Sect, Escola Paulista Med, BR-04044020 São Paulo, BrazilWeb of Scienc