Praziquantel and albendazole damaging action on in vitro developing Mesocestoides corti (Platyhelminthes : Cestoda)

Parasitic flatworms present several steps of body architecture rearrangement during their fast transition from one developmental stage to another, which are, at least in part, responsible for their evasion from host immune response. Besides, different developmental stages present different degrees of susceptibility to drug action, and the identification of more susceptible stages is of importance for the definition of therapeutical approaches. Mesocastoides corti (syn. Mesocestoides vogae) is considered a good model to Study cestode biology because it can be easily manipulated both in vivo and in vitro and due to its relatively close relationship to cestodes of medical relevance, such as those from genera Echinococcus or Taenia. We have analyzed the damaging action of two broad spectrum anthelmintic drugs (praziquantel and albendazole) throughout the in vitro strobilization process of M. corti in order to identify developmental stages or body structures more Susceptible to these drugs. Tetrathyridia (larval stage) and segmented-induced worms were cultivated and treated with praziquantel and albendazole. Whole mounted samples, taken from different developmental stages, were fixed and stained with fluorophore-labeled WGA lectin and phalloidin for the analysis of tegument and Muscles, respectively. Conflocal laser scanning microscopy was used to identify anatomical changes and lesions caused by each anthelmintic drug in a 3D view. We demonstrated that both praziquantel and albendazole cause extensive tissue damage, especially on tegument, and that adult forms were the most Susceptible to drug exposure. (c) 2005 Elsevier Ireland Ltd. All rights reserved.Univ Fed Rio Grande Sul, Ctr Biotechnol, LMBC, BR-91501970 Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, Disciplina Biol Mol, São Paulo, BrazilUniv Chile, Fac Med, ICBM, Mol Biol Lab, Santiago 7, ChileUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, Disciplina Biol Mol, São Paulo, BrazilWeb of Scienc