cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients

Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with development of peptic ulcer disease and gastric malignancies. the vacuolating cytotoxin (vacA), cagA gene, and babA2 gene are important virulence factor involving gastric diseases. Eighty-nine Helicobacter pylori-positive gastric biopsies were analyzed by polymerase chain reaction and Southern blotting for H. pylori detection and genotyping with primer pairs from each virulence gene. Fifty-three strains (59%) were common vacA genotype s1/m1, and only 14 (16%) were s2/m2, 12% of strains was found to have multiple infection. the cagA presence was detected in 48% (43 strains) and babA2 gene was detected in 44% of our H. pylori strains. We observed high percentage of s1/m2 strains with chronic gastritis and peptic ulcer and a significant correlation between cagA presence with the s1 allele and babA2 gene with chronic gastritis. (c) 2005 Elsevier Inc. All rights reserved.Fac Med Marilia, FAMEMA, Homoctr, Disciplina Genet, BR-17519050 Marilia, SP, BrazilFac Med Marilia, FAMEMA, Homoctr, Disciplina Biol Mol, BR-17519050 Marilia, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Genet, BR-04023900 São Paulo, BrazilHosp Sirio Libanes, Lab Patol Mol, BR-01308050 São Paulo, BrazilFac Med Marilia, FAMEMA, Disciplina Cirurg, BR-17519050 Marilia, SP, BrazilFac Med Marilia, Serv Endoscopia, BR-17519050 Marilia, SP, BrazilFac Med Marilia, FAMEMA, Disciplina Patol, BR-17519050 Marilia, SP, BrazilUniv São Paulo Bauru, BR-17011160 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Genet, BR-04023900 São Paulo, BrazilWeb of Scienc