Dual effect of agmatine in the bisected rat vas deferens

The functional effects of the amine agmatine, the putative endogenous ligand for alpha(2)-adrenoceptors and imidazoline receptors, in rat vas deferens were investigated by using the epididymal and prostatic portions. Tissues were contracted by electrical stimulation or by exogenous drugs. in electrically stimulated portions, agmatine caused a dual effect on contractions. in the epididymal portion an inhibition on twitch contractions was observed, which was partially antagonised by idazoxan and yohimbine, indicating the involvement of at least a presynaptic alpha(2)-adrenoceptor-mediated mechanism, without the interference of imidazoline receptors. in the prostatic portion, agmatine enhanced the amplitude of twitches. in contractions induced by exogenous drugs, agmatine potentiated, only in the prostatic segment, the effects of noradrenaline (norepinephrine) or ATP; it also enhanced the effect of low concentrations of KCl and blocked the maximum effect of the higher concentrations. Effects induced by agmatine on the exogenous ATP in the prostatic portion were blocked by cromakalim, suggesting a blocking action on the postsynaptic K+ channels, which explains, in part, the potentiation of the twitch amplitude. It was concluded that agmatine interferes with sympathetic neurotransmission, but the physiological relevance of this needs to be better understood because of the high doses employed to induce its effects.Univ Fed Fluminense, Fac Farm, Dept Farm & Adm Farmaceut, BR-24241000 Niteroi, RJ, BrazilUNIFESP, Escola Paulista Med, Dept Farmacol, BR-04021023 São Paulo, BrazilUNIFESP, Escola Paulista Med, Dept Fisiol, BR-04021023 São Paulo, BrazilUNIFESP, Escola Paulista Med, Dept Farmacol, BR-04021023 São Paulo, BrazilUNIFESP, Escola Paulista Med, Dept Fisiol, BR-04021023 São Paulo, BrazilWeb of Scienc