Conditioned immunosuppression of lipopolysaccharide-induced antibody response of orally immunized mice

BALB/C mice previously conditioned in a paired regimen in which cycophosphamide was given in association with a novel taste in the drinking water (saccharin, SAC) subsequently received LPS (1mg/mouse) by oral gavage. Subgroups of mice were reexposed to SAC or water respectively, and anti-LPS antibody production measured 6 days later using an ELISA/ELISPOT assay. Our data show that the immune response to this T-independent antigen, administered orally, is amenable to conditioned immunosuppression in this model system. (C) 1997 Academic Press.Universidade Federal de São Paulo,ESCOLA PAULISTA MED,DEPT PEDIAT,São Paulo,BRAZILUNIV TORONTO,DEPT SURG & IMMUNOL,TORONTO,ON,CANADAUniversidade Federal de São Paulo,ESCOLA PAULISTA MED,DEPT PEDIAT,São Paulo,BRAZILWeb of Scienc