Add to ORKGThrough screening of DNA polymerase libraries that contain arbitrary randomized mutants generated by epPCR, we were able to identify enzymes that exhibit RT-PCR function, a function that is imperceptible in the wildtype enzyme. As demonstrated, the identified mutants might find immediate applications and provide the basis for the development of new means for single-step RT-PCR technologies like pathogen RNA detection or gene expression analysis in real time
Background: Restriction endonuclease-mediated selective (REMS)-PCR, allows detection of point mutati...
Directed evolution plays a crucial role in advancing modern biotechnological, medical, and industria...
Natural enzymes, when used in biotechnological applications are often found not well suited for th...
Through screening of DNA polymerase libraries that contain arbitrary randomized mutants generated by...
Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcr...
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. D...
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. D...
DNA polymerases the key enzymes for several biotechnological applications. Obviously, nature has not...
<div><p>Viral metagenomic libraries are a promising but previously untapped source of new reagent en...
DNA polymerases are involved in all DNA synthesis occurring in nature. Furthermore, DNA polymerases ...
International audienceWe describe the cloning and characterisation of a mutated thermostable DNA pol...
Engineered enzymes are of increasing importance for a plethora of biotechnical applications. Especia...
The ability to synthesize and propagate genetic information encoded in the framework of xeno-nucleic...
Nucleic acid polymerases are the most important reagents in biotechnology. Unfortunately, their high...
In this PhD thesis, several projects about the functional analysis and recruitment of mutated DNA po...
Background: Restriction endonuclease-mediated selective (REMS)-PCR, allows detection of point mutati...
Directed evolution plays a crucial role in advancing modern biotechnological, medical, and industria...
Natural enzymes, when used in biotechnological applications are often found not well suited for th...
Through screening of DNA polymerase libraries that contain arbitrary randomized mutants generated by...
Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcr...
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. D...
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. D...
DNA polymerases the key enzymes for several biotechnological applications. Obviously, nature has not...
<div><p>Viral metagenomic libraries are a promising but previously untapped source of new reagent en...
DNA polymerases are involved in all DNA synthesis occurring in nature. Furthermore, DNA polymerases ...
International audienceWe describe the cloning and characterisation of a mutated thermostable DNA pol...
Engineered enzymes are of increasing importance for a plethora of biotechnical applications. Especia...
The ability to synthesize and propagate genetic information encoded in the framework of xeno-nucleic...
Nucleic acid polymerases are the most important reagents in biotechnology. Unfortunately, their high...
In this PhD thesis, several projects about the functional analysis and recruitment of mutated DNA po...
Background: Restriction endonuclease-mediated selective (REMS)-PCR, allows detection of point mutati...
Directed evolution plays a crucial role in advancing modern biotechnological, medical, and industria...
Natural enzymes, when used in biotechnological applications are often found not well suited for th...