A bagasse-hy drolyzing enzyme of Aspergillus awamori (Department of Agricultural Chemistry)

バガスを唯一炭素源とした無機塩培地で培養した泡盛麹菌によるバガス分解酵素の生産について調べ, 次の結果を得た。泡盛麹菌22株をアルカリ処理バガス含有培地で培養したところ, そのほとんどが培養液中にアルカリ処理バガスを分解する酵素を生産した。供試菌株で酵素生産が高かったAsp.awamori IFO 4033を用い, 酵素生産を向上させるための培養条件の検討を行なった。本菌株を0.5%アルカリ溶液を用いて120℃, 20分間処理したバガスを含む培地で培養した場合に最高の酵素生産が得られた。培地に加えるバガスの濃度は1.5∿2.0%が適当であった。This paper reports the production of a bagasse hydrolyzing enzyme by Aspergillus awamori grown on a mineral medium containing sugarcane bagasse as the sole carbon source. The results obtained were as follows : The enzyme production was investigated with 22 strains of Asp. awamori. All of them produced the enzyme in the culture broth of a medium containing alkali-treated bagasse. Among them, Asp. awamori IFO 4033 showed the highest production of the enzyme. In order to find the medium conditions required for the improvement of the enzyme production by this strain, the organism was grown in a medium containing the bagasse treated in various conditions. The best enzyme production was obtained when the organism was grown in a medium containing the bagasse treated with 0.5% NaOH solution at 120℃ for 20min. A medium containing 1.5-2.0% of the above alkalitreated bagasse gave the best result for the enzyme production