Limited impacts of experimental flow releases on water quality and macroinvertebrate community composition in an upland regulated river

River regulation following the construction of dams has affected the hydrology, water quality, and biology of watercourses across the globe. The term “environmental flows” has been used to describe measures that can be employed to return some lost elements of the natural flow regime. Their introduction has been suggested as a way to mitigate the impacts of river regulation throughout the world, but understanding of the effects of artificial high flows on water quality and biota is limited for many different river types. We report a field study that manipulated compensation flows from reservoirs in the Pennine uplands of northern England and measured changes in water quality and benthic macroinvertebrates using a before‐after‐control‐impact approach. These resulted in minor short‐term changes in water quality, but there was no evidence of immediate (within 48 hr) responses by the macroinvertebrate community to individual flow releases. However, a shift in macroinvertebrate community composition was found after multiple releases, characterized by reductions in Amphinemura sulcicollis (Plecoptera) and Baetis rhodani (Ephemeroptera) and changes in the density of all Diptera. The introduction of short‐term flow pulses in flashy regulated river systems is unlikely to yield significant changes in water quality and biota. Nevertheless, cumulative rather than single environmental flow events show promise for mitigating some of the impacts of river regulation. More widely, our findings indicate that environmental flow releases from reservoirs may have to go beyond occasional experimental high flow releases if these rivers are to more closely mimic unregulated river systems