Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases.
- Huang, K
- Parmeggiani, F
- Ledru, H
- Hollingsworth, K
- Mas Pons, J
- Marchesi, A
- Both, P
- Mattey, AP
- Pallister, E
- Bulmer, GS
- van Munster, JM
- Turnbull, WB
- Galan, MC
- Flitsch, SL
DOIAbstract
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst
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