The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vertebrate host macrophages. A recent model suggests that promastigote and amastigote forms of the parasite mimic mammalian apoptotic cells by exposing phosphatidylserine (PS) at the cell surface to trigger their phagocytic uptake into host macrophages. PS presentation at the cell surface is typically analyzed using fluorescence-labeled annexin V. Here we show that Leishmania promastigotes can be stained by fluorescence-labeled annexin V upon permeabilization or miltefosine treatment. However, combined lipid analysis by thin-layer chromatography, mass spectrometry and 31 P nuclear magnetic resonance (NMR) spectroscopy revealed that Leishmania pro...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
<div><p>The protozoan parasite <em>Leishmania</em> is an intracellular pathogen infecting and replic...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
<div><p>The protozoan parasite <em>Leishmania</em> is an intracellular pathogen infecting and replic...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
The protozoan parasite Leishmania is an intracellular pathogen infecting and replicating inside vert...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...
Phosphatidylcholine (PC) is the most abundant type of phospholipids in eukaryotes constituting ~30% ...