MICROSATELLITE LOCI FOR TUCUMA OF AMAZONAS (ASTROCARYUM ACULEATUM) AND AMPLIFICATION IN OTHER ARECACEAE

Premise of the study: Microsatellite loci were developed for tucuma of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucuma of Amazonas (Manaus and Manicore cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucuma.Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)Financiadora de Estudos e Projetos (FINEP)Financiadora de Estudos e Projetos (FINEP)FAPEAM [01.06.0380.04]FAPEAMConselho Nacional de Pesquisa e Desenvolvimento (CNPq) [553254/2005-7]Conselho Nacional de Pesquisa e Desenvolvimento (CNPq)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Programa de Excelencia Academica (Proex) of the Departamento de Genetica ESALQUSPCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)-Programa de Excelencia Academica (Proex) of the Departamento de Genetica ESALQ-US