The malaria parasite can synthesize haem de novo. In the present study, the expression of the parasite gene for δ-aminolaevulinate synthase (PfALAS) has been studied by reverse transcriptase PCR analysis of the mRNA, protein expression using antibodies to the recombinant protein expressed in Escherichia coli and assay of ALAS enzyme activity in Plasmodium falciparum in culture. The gene is expressed through all stages of intra-erythrocytic parasite growth, with a small increase during the trophozoite stage. Antibodies to the erythrocyte ALAS do not cross-react with the parasite enzyme and vice versa. The recombinant enzyme activity is inhibited by ethanolamine and the latter inhibits haem synthesis in P. falciparum and growth in culture. Th...
A number of antimalarial drugs interfere with the electron transport chain and heme-related reaction...
The parasite Plasmodium berghei imports the enzyme $\delta$-aminolevulinate dehydratase (ALAD), and ...
The combination of drug resistance, lack of an effective vaccine, and ongoing conflict and poverty m...
The malaria parasite can synthesize haem de novo. In the present study, the expression of the parasi...
The malaria parasite can synthesize haem de novo. In the present study, the expression of the parasi...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host $\delt...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host δ-amin...
Our previous studies have demonstrated de novo haem biosynthesis in the malarial parasite (Plasmodiu...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host δ...
The mouse and human malarial parasites, Plasmodium berghei and Plasmodium falciparum, respectively, ...
Heme metabolism is central to malaria parasite biology. The parasite acquires heme from host hemoglo...
The malarial parasite manifests unique features of heme metabolism. In the intraerythrocyte stage it...
Plasmodium falciparum is a parasite that causes severe type of malaria, including cerebral malaria. ...
The malarial parasite manifests unique features of heme metabolism. In the intraerythrocyte stage it...
All eight enzymes required for de novo heme biosynthesis have been predicted from the nuclear genome...
A number of antimalarial drugs interfere with the electron transport chain and heme-related reaction...
The parasite Plasmodium berghei imports the enzyme $\delta$-aminolevulinate dehydratase (ALAD), and ...
The combination of drug resistance, lack of an effective vaccine, and ongoing conflict and poverty m...
The malaria parasite can synthesize haem de novo. In the present study, the expression of the parasi...
The malaria parasite can synthesize haem de novo. In the present study, the expression of the parasi...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host $\delt...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host δ-amin...
Our previous studies have demonstrated de novo haem biosynthesis in the malarial parasite (Plasmodiu...
The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host δ...
The mouse and human malarial parasites, Plasmodium berghei and Plasmodium falciparum, respectively, ...
Heme metabolism is central to malaria parasite biology. The parasite acquires heme from host hemoglo...
The malarial parasite manifests unique features of heme metabolism. In the intraerythrocyte stage it...
Plasmodium falciparum is a parasite that causes severe type of malaria, including cerebral malaria. ...
The malarial parasite manifests unique features of heme metabolism. In the intraerythrocyte stage it...
All eight enzymes required for de novo heme biosynthesis have been predicted from the nuclear genome...
A number of antimalarial drugs interfere with the electron transport chain and heme-related reaction...
The parasite Plasmodium berghei imports the enzyme $\delta$-aminolevulinate dehydratase (ALAD), and ...
The combination of drug resistance, lack of an effective vaccine, and ongoing conflict and poverty m...