Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase
- Dinamarco, Taisa Magnani
- Brown, Neil Andrew
- Couto de Almeida, Ricardo Sergio
- Castro, Patricia Alves de
- Savoldi, Marcela
- Souza Goldman, Maria Helena de
- Goldman, Gustavo Henrique
DOIAbstract
The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken to...
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