The Snake Venom Peptide Bj-PRO-7a Is a M1 Muscarinic Acetylcholine Receptor Agonist

NEGRAES, Priscilla D.
LAMEU, Claudiana
HAYASHI, Mirian A. F.
MELO, Robson L.
CAMARGO, Antonio C. M.
ULRICH, Henning

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Publication date

January 2011

DOI

10.1002/cyto.a.20963

Publisher

WILEY-BLACKWELL

Journal

1552-4922

Abstract

Proline-rich peptides from Bothrops jararaca venom (Bj-PRO) were characterized based on the capability to inhibit the somatic angiotensin-converting enzyme. The pharmacological action of these peptides resulted in the development of Captopril, one of the best examples of a target-driven drug discovery for treatment of hypertension. However, biochemical and biological properties of Bj-PROs were not completely elucidated yet, and many recent studies have suggested that their activity relies on angiotensin-converting enzyme-independent mechanisms. Here, we show that Bj-PRO-7a (<EDGPIPP) specifically activates [Ca(2+)](i) transients in CHO-M1 cells expressing heterologous rat M1 muscarinic subtype. The activation curve established by microfluor...