Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent α-glycerol-3-phosphate dehydrogenase

This work reports the primary nucleotide structure and in vitro translation of a cDNA, expressed by a gene mapping on chromosome 12, that encodes a human hepatic α-glycerol-3-phosphate dehydrogenase (L-glycerol-3-phosphate:NAD oxidoreductase, E.C. 1.1.1.8). The 1413 bp cDNA comprises an ORF of 1050 bp that encodes a 349 amino acid protein of 37.5 kDa. Northern blot analysis of poly(A)+ mRNA from human liver showed three transcripts, while from human placenta only two transcripts were detected.This work has been supported in part by grants from the Spanish Plan Nacional de Investigación Científica y Técnica (SAL 509/91 and SAF 93-0788), Dirección General de Investigación Científica y Técnica (PB93-0163) and Fondo de Investigaciones Sanitarias (93/0162). J.M. was supported by the Spanish Ministry of Science and Education and C.G.-M. by a joined action from Spanish Council of Research (CSIC) and Fundación Ramón Areces