Reference ranges of Presepsin in preterm infants in the first 48 h of life : A multicenter observational study

Background and objectives: Despite low incidence of early-onset sepsis, the majority of preterm newborns receive early antibiotic prophylaxis. Our aim was to assess reference ranges of Presepsin during the first 48 h of life in preterm infants and to evaluate the possible influence of neonatal and maternal factors on its values. Methods: This observational study consecutively enrolled newborns 64 32 weeks of GA at 0\u20136 h of life. Enrolled infants were sampled for blood culture, complete white blood cell (WBC) count, and P-SEP at 0\u20136 (T0) h of life and for P-SEP at 12 \ub1 3 (T1), 24 \ub1 3 (T2), and 48 \ub1 6 (T3) h of life. Cases of EOS were not considered for the statistical analysis. Results: Data analysis was performed for 183 patients. P-SEP median values were 583 ng/L at T0 (IQR 405\u2013800 ng/L, 5th and 95th percentiles 204 and 1200 ng/L), 614 ng/L at T1 (IQR 450\u2013812 ng/L, 5th and 95th percentiles 262 and 1171 ng/L), 604 ng/L at T2 (IQR 445\u2013825 ng/L, 5th and 95th percentiles 292 and 1302 ng/L) and 513 ng/L at T3 (IQR 371\u2013734 ng/L, 5th and 95th percentiles 249 and 1278 ng/L). P-SEP values are negatively associated to gestational age (GA) at T0, T1, and T2. Conclusions: We determined for the first time the reference ranges of P-SEP during the first 48 h of life in very preterm infants and provided its percentile distribution at T0, T1, T2 and T3. P-SEP measurement was affected by GA at T0, T1, T2