One of the under-utilised potential agricultural sources of bioenergy feedstock is the herbaceous biomass obtained from semi-natural grasslands. For biodiversity maintenance, it is important that semi-natural grasslands are managed continuously using extensive methods (one cut per year and without seeding, fertilisation). However, many countries are facing great difficulties with consuming the biomass from these grasslands due to its low forage value. Therefore finding an alternative use of biomass from semi-natural grasslands has become a challenging option for bioenergy production. The main aim of the thesis is to evaluate the energy potential of herbaceous biomass from three Estonian semi-natural grassland types (alluvial meadow, dry to ...