J Occup Environ Hyg

The objective of this study was to investigate the efficacy of an aerodynamic separation scheme for obtaining aerosols with nearly monodisperse fiber lengths as test samples for mechanistic toxicological evaluations. The approach involved the separation of aerosolized glass fibers using an Aerodynamic Aerosol Classifier (AAC) or a multi-cyclone sampling array, followed by the collection of separated samples on filter substrates, and the measurement of each sample fiber length distribution. A glass fiber aerosol with a narrow range of aerodynamic sizes was selected and sampled with the AAC or multi-cyclone sampling array in two separate setups. The fiber length and diameter were measured using a field emission scanning electron microscope. The glass fiber aerosol was separated in distinct groups of eight with the AAC and of four with the multi-cyclone sampling array. The geometric standard deviations of the fiber length distributions of the separated aerosols ranged from 1.49 to 1.69 for the AAC and from 1.6 to 1.8 for multi-cyclone sampling array. While the separation of glass fiber aerosols with an AAC is likely to produce two different length fiber groups and the length resolution may be acceptable, the overall mass throughput of these separation schemes is limited.20202020-09-04T00:00:00ZCC999999/ImCDC/Intramural CDC HHS/United States32294024PMC7473384832