Spectrophotometric determination of iron in a pharmaceutical : bachelor thesis

Željezo je metal poznat i koristan čovjeku, kako u tehničkom smislu tako i u nutritivnom. Jedan je od važnih bioelemenata našeg organizma. Zaslužan je za opskrbu kisikom i normalno funkcioniranje organizma. Njegov nedostatak uzrokuje slabokrvnost ili anemiju, koja se može liječiti prehranom ili lijekovima. U ovom eksperimentalnom radu je opisano spektrofotometrijsko određivanje željeza u lijeku Tardyferon namijenjenom za liječenje anemije, koji je prethodno raščinjen u mikrovalnoj pećnici. UV/VIS spektroskopija je metoda koja se primjenjuje za određivanje nepoznate koncentracije uzorka pomoću Beerovog zakona. Mjerenje se vršilo u vidljivom području pri valnoj duljini 506 nm i kiseloj sredini pH=1.Iron is metal well known and useful for human, as in technical way and in nutritional way. It is one of important bioelement of our system. It is responsible for the oxygen supply and for the normal functioning of the organism. Its lack in organism causes anemia which can be treated with food or pharmaceutical. Spectrophotometric determination of iron in pharmaceutical Tardyferon which is intended to treat anemia, is described in this experimental work. Preparation is previously dissolved in microwave oven. UV/VIS spectroscopy is method which is used to determine an unknow sample concentration using Beer law. The measurement was performed in the visible range at the wavelength of 506 nm and acidic enviroment at pH=1