OCT-based reconstruction of the crystalline lens Gradient Refractive index: changes with age and accommodation

Conferencia invitada; Orlando, United States, 4–8 May 2014.The optical properties of the crystalline lens are largely determined by its shape and its gradient index (GRIN) distribution. Knowledge of their change of these properties with accommodation and age will give us important insights into the mechanism of accommodation and presbyopia. Quantitative Optical Coherence Tomography (OCT) allowed measurements of the 3-D shape the lens in vivo and in vitro. In addition, an OCTbased method, combined with global search algorithms allowed reconstruction of the crystalline lens GRIN in vitro. Studies were performed in porcine lenses, human lenses of different ages and nonhuman primate lenses under simulated accommodation in a stretcher system. Experimental measurements of shape and GRIN in the same lenses allowed the first direct a nalysis of their relative contribution to the crystalline lens sphericalEuropean Research Council ERC-AdG-294099; Spanish Government FIS2011-25637; CSIC i- LINK+0609Peer Reviewe