Structural rearrangements detected in newly-formed hexaploid tritordeum after three sequential FISH experiments with repetitive DNA sequences

Mitotic chromosomes of tritordeum (HchHchAABB; 2n = 6x = 42; HT27) and their respective parents, Hordeum chilense L. (HchHch; 2n = 2x = 14; H7) and Triticum turgidum (AABB; 2n = 4x = 28; T81), were analysed after three sequential fluorescence in situ hybridization (FISH) experiments performed with six probes: the simple sequence repeats (SSRs) (AG)10 and (AAC)5; genomic DNA from H. chilense; 45S rDNA (pTa71); 5S rDNA (pTa794); and pSc119.2, to achieve a comparative analysis about the distribution of these sequences in the three plant materials. The SSR (AG)10 probe showed eight hybridization signals on tritordeum chromosomes and six in the chromosomes of its male parent (durum wheat). No hybridization signals were detected in female parent H. chilense.This work was partially supported by the HY-WHEAT project, International Consortium (P-KBBE/AGRGPL/0002/2010), funded by COMPETE, QREN, EU and the Portuguese Foundation for Science and the Technology (FCT).Peer Reviewe