Multi-parametric evaluation of autologous cultivated Limbal epithelial cell transplantation outcomes of Limbal stem cell deficiency due to chemical burn

Gurdal, Mehmet/0000-0003-1226-1737WOS: 000561115700003PubMed: 32762738Background the sparsity of established tools for the grading of limbal stem cell deficiency hinder objective assessments of the clinical outcome of cultivated limbal epithelial cell transplantation. To advance towards the development of standards for the comparison of the outcomes of these bio-surgical protocols we have now applied a battery of recognized objective and patient-declared subjective outcome criteria to the autologous modality of cultivated limbal epithelial cell transplantation. Methods the prospective study involved ten patients (M/F = 9/1; mean age = 42.1 years) displaying overt unilateral limbal stem cell deficiency complying with the inclusion criteria described in Methods. Limbal biopsies were obtained from the contralateral eye and their outgrowths after 2-week cultures were transplanted on the affected eye after pannus resection. Outcomes were followed up for 12 months. the objective tests were scores for best-corrected visual acuity (BCVA); using the LogMAR scale, a multiparametric ocular surface score (OSS), and the Schirmer's test. Subjective scores were based on patient answers to a) perception of visual improvement/pain; b) the 25-item National Eye Institute Visual Function Questionnaire (NEI-VFQ 25); and c) the 12-item Ocular Surface Disease Index Questionnaire (OSDI). All procedures were performed under good manufacture practices using solely xeno-free reagents. in all cases, a single biopsy was divided into two pieces and they were expanded in order to prevent outgrowth failure. in 5 patients, both biopsies generated healthy culture sheet. in those cases the lesser outgrowth were used for immune-histological characterization. Results the experimental parallel outgrowth samples showed a similar percent of p63 alpha(+)cells. PreOp and 12-month PostOp BCVAs and OSSs were, respectively, 1.15 +/- 0.70; 0.21 +/- 0.13 and 7.40 +/- 2.01; 2,30 +/- 1.30, (p < 0.05). Patient's responses to all three question sets except ocular pain were consistent with significant improvement (p < 0.05). Conclusion Objective clinical metrics demonstrate that in patients with limbal stem cell deficiency, cultivated limbal epithelial cell transplantation improves vision and ocular surface health and subjective visual perceptions.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115S423]; USPHSUnited States Public Health Service [RO1-EY 029279, RO1-EY014878]OBS: the Scientific and Technological Research Council of Turkey (TUBITAK)(Project number:115S423); JMW: USPHS RO1-EY 029279 and RO1-EY014878