Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise

It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP),we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protein basis inhuman skeletal muscle. Age-matched, overweight males consumed9 d of a high-fat, low-carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein by-protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle