A new Pyrenean hybrid Cirsium (Asteraceae) as revealed by morphological and molecular analyses

14 páginas, 3 figuras, 4 tablas.A new nothosubspecies Cirsium × vivantii is described after a molecular and morphological characterization of eight individuals collected in two separate valleys of the French central Pyrenees. Both hypervariable Amplified Fragment Length Polymorphism (AFLP) markers and nuclear rDNA (ITS) and plastid (trnL-F, TRNT-L) DNA sequences were analysed. The profiles of these hybrid samples were compared to those of 43 individuals belonging to their presumed parental taxa C. carniolicum ssp. rufescens and C. palustre. A total of 133 AFLP bands were scored from three primer-pair combinations. All 130 AFLP bands that amplified in the hybrid samples were present in either C. carniolicum ssp. rufescens, C. palustre or both taxa, supporting the participation of these plant genomes in the resulting hybrids. Several Additive Polymorphic Sites (APS) detected in the ITS sequences of the hybrid samples also confirmed their derived origins from ribotypes of the two parental taxa. The lack of exclusive AFLP markers and the nonconcerted evolution of rDNA polymorphisms towards either of the parental ribotypes indicated their recent origin. Plastid trnT-L sequences were used to identify C. palustre as the maternal parent of six of these hybrid individuals; either parent could have acted as the plastid genome donor for the other two individuals. The morphological study revealed that all hybrid individuals were morphologically intermediate between their parents showing largely lobed and less spiny basal leaves as in C. carniolicum ssp. rufescens and decurrent leaf bases and pinkish corollas as in C. palustre.This study was supported by a Spanish Aragón Government grant project B39 (Bioflora) to P. Catalán, L. Villar, J.G. Segarra- Moragues and E. Pérez-Collazos. J. López and L. Villar were subsidized by the Spanish Ministry of Science grant project Flora Iberica VI and VII (D.G.I.C.Y.T) (REN2002-04634-C05-04 and CGL2005-05471-CO4). J.G. Segarra-Moragues was partially supported by a postdoctoral I3P grant from the Consejo Superior de Investigaciones Científicas (CSIC).Peer reviewe