Non-destructive measurement of canopy cover is an alternative to biomass sampling at anthesis to predict yield of canola-quality Brassica juncea

Abstract Biomass at anthesis is an important trait in predicting yield of Brassica oilseeds in environments where seed filling occurs in dry and warm conditions. This study aimed to compare the ability of non-destructive canopy cover measurements (Sunfleck ceptometer and digital photography) with direct biomass sampling at anthesis to predict the yield of canola-quality B. juncea (juncea canola) hybrids. Field experiments were conducted in the Victorian Mallee (226-248 mm annual rainfall) and the Wimmera (266-407 mm annual rainfall) regions from 2012 to 2014. Nineteen juncea canola genotypes were sown in the first year and 10 to 11 genotypes in the subsequent two years of field experiments. The experimental plots were laid out in a randomized complete block design with three replications. Days to 50% flowering, canopy cover and biomass at 50% flowering and seed yield were recorded. The study concluded that at low rainfall sites (<250 mm annual rainfall), the canopy cover measurements had consistent and significant positive relationships with biomass at anthesis (r 2 =0.43-0.61 in 2012 and r 2 =0.72 in 2013) and seed yield (r 2 =0.25-0.41 in 2012 and r 2 =0.51 in 2013). Canopy cover also showed a positive and significant relationship with early flowering (r 2 =0.52 in 2012 and r 2 =0.60 in 2013) at the relatively low rainfall site. These results suggest that non-destructive canopy cover measurement could replace direct biomass sampling at anthesis in prediction of yield of juncea canola hybrids in low rainfall environments