PRODUCTON OF DOUBLE HAPLOID POPULATION IN TWO INDICA RICE (Orysa sativa L.) CROSS SAFRI-17XIR-64 AND MTU1010 VARIETY

Abstract: Anther culture based double haploid (DH) production is a technology which can significantly reduce the time period required for development of new crop variety. In the present investigation an attempt was made to develop DH using anther culture in rice (Oryza sativa L.). One cross Safri-17xIR-64 and one variety MTU1010 was subject for the study. Parameter like media composition, hormonal treatment etc was standardized for efficient development of DH. The anther were excised and plated on to N6 (Chu.,1978) media supplemented with 3% maltose 0.8% agar , 2 ml/l 2,4-D and the pH was maintained of 5.8. In cross Safri-17xIR64 the callus induction percent was 0.49% and in variety MTU1010 callus induction percent is 0.40%.The induce callus was transfer in 16 different media (T1 to T16 ) for regeneration. Then green callus was transfer to green plant regeneration media. Treatment no.T15 was found to be best as it has produce 106 number of green plant & treatment no.T1 has produced 58 albino plants in Safri17xIR-64. In MTU1010, T1 has generated 9 green plants and 5 albino plants