Prediction of Potentially Unstable Electrical Activity during Embryonic Development of Rodent Ventricular Myocytes

Abstract In order to evaluate developmental changes in embryonic ventricular cells at early embryonic (EE) and late embryonic (LE) stage, we aimed to predict potentially unstable action potentials (APs) that could be lethal to developing ventricular cells. Two models of the Introduction In rodent, the electrophysiological properties of individual ion channels have been investigated in isolated ventricular myocytes at the representative 4 stages, early embryonic (EE), late embryonic (LE), neonatal, and adult, via patch clamp methods Jonsson et al. (2012) combined molecular biology and computer simulation to demonstrate that human embryonic stem cell-derived cardiomyocytes (hESC-CM) have an immature electrophysiological phenotype Methods We utilized the two mathematical models, the Kyoto and Luo-Rudy model, in which the relative activities of ionic components were switched independently between EE and LE stages to represent different combinations of the components in embryonic guinea pig ventricular cells. Embryonic ventricular cell models Previously, we simulated the APs of rodent ventricular cells at the EE, LE stages using the Kyoto model, an electrophysiological model of guinea pig ventricular cells Switching stages of ionic components We selected the 9 components to be switched between the EE and LE stages: funny current (I f ), sustained inward current (I st ), inward rectifier current (I K1 ), Na + current (I Na ), L