LACTATE DEHYDROGENASE STUDIES ON THE TESTIS OF SIALOADENECTOMISED MOUSE RECEIVING ISOPROTERENOL

Abstract: Male albino mice (Mus musculus) of the age 1 month and weight (9 to 15 gms) were selected for the present work. Animals, subjected to sublingulectomy and sialoadenectomy, were divided into six groups. Group-I (control), Group-II (sublingulectomised), Group-III (sialoadenectomised), Group-IV (sialoadenectomised submandibular gland extract administered), Group-V (isoproterenol administered), Group-VI (sialoadenectomised isoproterenol administered). The animals were kept for two months. Thereafter, the animals were sacrificed and testis were removed and used for the estimation of lactate dehydrogenase (LDH) by biochemical and polyacrylamide gel electrophoresis methods. Study shows LDH activity in testes of groups II and III was decreased significantly as compared to group I, but increased significantly in Group IV as compared to group III. Study reveals significantly increased activity in group V, but not in group VI as compared to group III. Electrophoretic separation of LDH in the testis of normal mice showed six clear bands i.e. LDH-I, II, III, IV, V, and LDH-X. In sublingulectomised, sialoadenectomised, sialoadenectomised submandibular gland extract administered mice LDH-X was missing. Intense activity was observed for LDH-X in the control mice receiving isoproterenol but not in the sialoadenectomised mice receiving isoproterenol