Comparative Genomic Studies in Leguminous Species

Abstract: Comparative Genomics is relatively a new discipline of Genetics, which deals with the relationships between the genomes of genera, species, varieties or strains. For elaborating comparative genomics of legumes; fourteen accessions belonging to seven species viz; Vigna radiate, Vigna mungo, Vicia ervilia, Vicia faba, Phaseolus vulgaris, Cicer arietinum and Lens culinaris, were analyzed using Randomly Amplified Polymorphic DNA (RAPD). Alleles of various sizes ranging from 500-1400 bp were amplified. On an average 3.5 allele per genotype were amplified. The average genetic distance ranged from 4-91%. Phylogenetic relationship among the legumes based upon DNA analysis was studied through dendrogram analysis. All the accessions were clustered in 6 groups. It was found that Vigna radiata and Vigna mungo accessions were most distantly related to each other