THE EFFECT OF DIFFERENT METHODS OF RHIZOBIUM BACTERIA INOCULATION ON BIOLOGICAL NITROGEN FIXATION IN BROAD BEAN

ABSTRACT: The experiment was carried out as a split plot in the form of randomized complete block design with four replications. Three main treatments included different applications of Rhizobium bacteria as E 0 : control (non-inoculation), E 1 : syrup, E 2 : gum Arabic. Three sub treatments included different broad bean cultivars as V 0 : Algerian, V 1 : Barekat, V 2: Shami, among the methods applied by the bacterium, inoculation with syrup was superior to other methods. The highest number of nodules belonged to inoculation treatment with syrup by 338.46 and the lowest belonged to the control (non-inoculation) by 89.38. Moreover, the highest number of nodules belonged to Barekat cultivar by 253.13 and the lowest number of nodules was observed in Algerian cultivar by 204.63. The highest number of roots was observed in inoculation with syrup by 143.81 and the lowest one belonged to the control treatment by 132.44 and also the highest number of roots belonged to Barekat cultivar by 141.99 and the lowest number belonged to Algerian cultivar. The highest volume of root in broad bean belonged to inoculation with syrup by 70.20 cc and the lowest volume of root belonged to the control treatment by 43.92 cc. in inoculation with syrup the highest rate of root dry weight was reported to be 84.72 g. the highest percent of nitrogen fixation was related to inoculation treatment with syrup by nearly 14% and the lowest percentage belonged to the control treatment (non-inoculation) by about 2%. In this experiment it became clear that inoculation method with syrup and Barekat cultivar were more effective and more efficient than other methods and cultivars which were applied