STUDIES ON SCREENING AND HISTOCHEMICAL LOCALISATION OF PHYTOCHEMICALS IN THE MEDICINAL PLANT BARLERIA LUPULINA LINDL

ABSTRACT The genus Barleria lupulina Lindl of the family Acanthaceae belongs to the sub tribe Barlerieae of the tribe Justicieae sensu Benth & Hook. The present investigation mainly emphasized on the histochemical localization of phytochemicals like alkaloids, starch, tannins, reducing sugars, proteins, flavonoids, amino acids and lignins. These localization were determined through colouristion using different reagents like Wagner's, Iodine Solutions, 10% Lead Acetate, Benedict's, Lugol's, 10% NaOH, Fehling's(A&B), Millon's, 0.2% Ninhydrin and 1% Phloroglucinol, The active compounds were identified prominently in different locations of the stem, leaf petiole and root of the medicinal plant B lupulina under study. It was found that presence of number of phytochemicals in xylem is higher than other tissues