V. 4. Absorption of Arsenate and Arsenite by Arsenic Hyperaccumlating Fern (Pteris vittata)

Phytoremediation using an arsenic hyperaccumulator, Pteris vittata L., has generated increasing interest worldwide, for it's both environmentally sound and cost effective. However, the mechanisms of arsenic uptake and accumulation by this plant are not clear at this time. This study shows the uptake of arsenate (As(V)) and arsenite (As(III)) using a hydroponic culture of P. vittata. The fern takes up both As(V) and As(III) from the culture which spiked with 50 mg of arsenic per litter, and can grow within 5 days of experimental period. Final amount of arsenic accumulated in the fern is 3.2 mg (As(V)), and 3.8 mg (As(III)). The submilli-scale distribution of elements by PIXE analysis clearly shows the accumulation of arsenic in fronds of P. vittata