Peer Review Antibacterial Activity of Binahong Rhizome Ethanol Extract (Anrederacordifolia (Tenore) Steen) Against Salmonella Typhi

Background. Anredera cordifolia is one of plants used as traditional medicine. It has been carried out a study about antibacterial activity of ethanolic extract of A. cordifolia rhizomes against Salmonella typhi and the phytochemical screening. The aims of this study are to find out the Minimum Bactericidal Concentration (MBC) of ethanolextract of A. cordifolia rhizomes against Salmonella typhi and to identify the chemical subtances of the ethanol extract found in the rhizomes. Method.The A. cordifoliarhizomes were extracted using maceration method with 70% v/v ethanol as solvent. The antibacterial activity was tested using liquid dilution method with varied concentration of the extract (50,49, 48, 47, 46, and 45% w/v) to determine MBC. Chromatography method was used to identify the chemical substances of the extract. Result. The result showed that the MBC of A. cordifolia rhizomes extract was 50% w/v. Theresult of the phytochemical screening with tube test and thin layer chromatography showed that theextract of Anredera cordifolia rhizomes contained flavonoids, polyphenols, and saponin. Conclusion.The binahong rhizome ethanol extract had antibacterial activity against S. typhi with 50% w/vof MBC and contained flavonoids, polyphenols, and saponin. Keywords : Anredera cordifolia, antibacterial, Salmonella typh