A comparative study of Cilnidipine and Telmisartan in tablets by high performance thin-layer chromatography with ultraviolet absorption densitometric detection

Background:Analysis of pharmaceutical compounds and newer drugs is commonly used in all the stages of drug discovery and development process.The present study was conducted to compare Cilnidipine and Telmisartan in tablets by high performance thin-layer chromatography with ultraviolet absorptiondensitometric detection.Materials & Methods:The present study was conducted on Cilnidipine and Telmisartan tablets. Separate HPTLC pre-coated plates of silica gel G 60 F254 (10x10) were employed for the spotting of standard solutions. 2ml of standard solutions of concentration 50,100, 200, 400, and 600mg/ml of Cilnidipine and Telmisartan standard solutions were applied in the five tracks respectively in two different plates.Results: Rf value for Cilnidipine was 0.46, height was 436.52 and area was 11834.2. Rf value for Telmisartan was 0.18, height was 372.74 and area was 2234.56. The accuracy of Cilnidipine was 98.12% and Telmisartan was 97.2%. Repeatability was 0.96 and 0.74 in cilnidine and Telmisartan respectively, intra-day value was 0.84-1.22 and 0.62-0.89 in cilnidine and Telmisartan respectively. Inter-day value was 0.92-1.36 and 1.24-1.48 in cilnidine and Telmisartan respectively. LOD was 12.8 ng/spot and 62.08 ng/spot in cilnidine and Telmisartan respectively. LOQ was 38.28 ng/spot and 185.0 ng/spot in cilnidine and Telmisartan respectively.Conclusion:Authors found that Rf value for Cilnidipine was 0.46 and for Telmisartan was 0.18. This method was found to be accurate and precise and canbe used for routine analysis of Cilnidipineand Telmisartan in pure and combined dosage form