Dielectrophoresis Based 3D Cell Rotation through Integration Of Bottom And Vertical Electrodes

Dielectrophoresis rotation of particles in 3D has important applications in biological cell manipulation, sorting and characterization. This paper reports the rotation of spherical bovine oocytes in yaw and pitch axes and their angular velocity spectrum by generating dielectric torque on the oocytes inside the chamber of a fabricated biochip. We predict by numerical computation and confirm experimentally that bovine oocytes are permanently polarizable with no Maxwell Wagner interfacial relaxation at critical frequency. The capability of rotating particles in 3D opens new research opportunities in cell manipulation and analysi