In vitro possibilities for vegetative multiplication of carnation (Dinathus sp.)

For in vitro regeneration of carnation (Dinathus sp.) as initial explants were used apical buds. The explants were cultivated on MS mineral solution, sucrose, agar-agar, inoslitol, casein, Vitamines B1, B6, nicotine acid and phytohormones: 0.5 mg/l IAA and 1 mg/l KIN. The cultures were kept under controlled conditions. Shoot of apical buds iniciated the hinges multiplication on MS medium with 0.5 mg/l IAA + 1 mg/l KIN + 0.5 mg/l BA. Rooting of shoots were obtained at 2 mg/l IAA + 0.1 mg/l KIN + 2 mg/l adenine. Rooted plants obtained from apical buds after 15-20 days in clime room conditions on a sterile mixture of pert, send and perlit (1:1:1) were will adapted. They normally grew and developed there