Co-electrospun gelatin-chondroitin sulfate/polycaprolactone nanofibrous scaffolds for cartilage tissue engineering

Chondroitin sulfate and gelatin (GEL-CS) as a component of cartilage extracellular matrix were incorporated by polycaprolactone (PCL)nanofibers utilizing co-electrospinning process to fabricate composite scaffolds for inducing chondrogenesis of human bone mesenchymal stem cells (hMSCs) differentiation without using differential medium. The MTT anSSEM results illustrated supported cellular attachment and viability of hMSCs on scaffolds. Sulfated glycosaminoglycan secretion staining, genes expression of COL2a1 and SOX9 and also type ⠡ collagen protein approved the differentiation of seeded hMSCs to chondrocytes without using any external chondrogenic differential factor. The scaffolds containing the highest GEL-CS content (2/1) exhibit better chondrogenesis differentiation results and it can be introduced as acceptable potential for cartilage tissue engineering application. © 2020 Elsevier Lt