High-density lipoproteins enhance progenitor-mediated endothelium repair in mice

Objective—We quantified endothelial progenitor cell (EPC) engraftment into the endothelial layer as an index of progenitor-mediated endothelial repair. Studies were conducted in C57BL/6J and in apolipoprotein E–deficient (apoE_/_) mice. We also investigated the possibility that high-density lipoproteins (HDL) may promote progenitormediated endothelial repair. Methods and Results—Thoracic aortic sections from C57BL/6J and apoE_/_ mice were analyzed for evidence of progenitor-derived endothelium as determined by the number of stem cell antigen-1–positive (Sca-1_) cells in the endothelial layer. EPCs (Sca-1_ cells) were significantly increased after endothelial damage induced by lipopolysaccharide (LPS) administration in C57BL/6J mice. The number of EPCs was greater in the aortic endothelium of untreated apoE_/_ than in untreated C57BL/6J mice and was similar to the number observed in LPS-treated C57BL/6J mice. The number of EPCs in the aortic endothelium of apoE_/_ mice more than doubled after intravenous infusion of reconstituted HDL. Conclusions—EPCs are recruited into the aortic endothelial layer of mice in response to an inflammatory insult. EPCs are also increased in the aortic endothelium of untreated apoE_/_ mice. The observation that number is further increased in apoE_/_ mice after injection of HDL suggests a role for HDL in promoting progenitor-mediated endothelial repair