Optimisation of Pueraria isoflavonoids by response surface methodology using ultrasonic-assisted extraction
- Wong, Ka H.
- Li, George Q.
- Li, Kong M.
- Razmovski-Naumovski, Valentina (R16317)
- Chan, Kelvin (R15157)
DOIAbstract
Puerariae Lobatae Radix (PLR) exerts cyto-protective effect against oxidative stress due to its high isoflavonoid content. In this study, the ultrasonic-assisted extraction condition for the maximum recovery of isoflavonoids with high cyto-protective effect was optimised by response surface methodology (RSM). A second-order polynomial fitted the experimental data (R2: 0.9736; p-value <0.0001). The optimal extraction parameters were determined as: extraction time 16.02 min, ethanol concentration 41.41% and liquid-to-solid ratio 44.35 mL/g. Practical experiments with extraction time 16.00 min, ethanol concentration 41.00% and liquid-to-solid ratio 44.00 mL/g were carried out in triplicate. This subsequently yielded a cell viability of 82.90 ±...
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