Aislamiento de bacterias nativas de Oncorhynchus mykiss con potencial probiótico frente a Yersinia ruckeri

The aim of this study was to identify native bacteria isolated from the intestinal tract of rainbow trout (Oncorhynchus mykiss) with probiotic properties against Yersinia ruckeri. In the study, 48 healthy trout from the hatchery of the Universidad Nacional del Altiplano (Puno, Peru) were used. The intestinal tract of the fry and juveniles was removed, and samples were taken from the intestinal mucosa. The samples were cultured on MRS agar and incubated at 30 °C for 24-48 h. The colonies were identified by their morphology and their inhibitory effect was evaluated in vitro against a Yersinia ruckeri strain isolated from a high mortality outbreak. Three isolates compatible with the genus Lactobacillus and one isolate compatible with the genus Bacillus were identified. These isolates showed inhibition halos greater than 9 mm in diameter against Y. ruckeri.  El objetivo del estudio fue identificar bacterias nativas aisladas del tracto intestinal de trucha arco iris (Oncorhynchus mykiss) con propiedades probióticas frente a Yersinia ruckeri. Se utilizaron 48 truchas sanas provenientes de la eclosería de la Universidad Nacional del Altiplano (Puno, Perú). El tracto intestinal de los alevines y juveniles fue extraído y se tomaron muestras de la mucosa intestinal. Las muestras fueron sembradas en agar MRS e incubadas a 30 °C por 24-48 h. Las colonias fueron identificadas por su morfología y se evaluó su efecto inhibitorio in vitro frente a una cepa de Yersinia ruckeri, aislada de un brote de alta mortalidad. Se identificaron tres aislados compatibles con el género Lactobacillus y un aislado compatible con el género Bacillus. Estos aislados mostraron halos de inhibición mayores a 9 mm de diámetro frente a Y. ruckeri. &nbsp